Laboratory Courses on Microbial culture

Welcome to the training course on Microbiological Methods! This course is designed to provide participants with a comprehensive understanding and practical skills in various microbiological techniques essential for research, industrial applications, and clinical diagnostics. Microbiology is a foundational discipline that plays a crucial role in fields such as medicine, agriculture, environmental science, and biotechnology.

Overview of Microbiological Methods

Microbiological methods encompass a wide range of techniques used to study microorganisms, including bacteria, viruses, fungi, and protozoa. These methods are essential for:

Microbial Identification and Characterization: Techniques such as staining, culture, and biochemical assays enable the identification and classification of microorganisms based on their morphological, physiological, and biochemical characteristics.
Microbial Culturing and Growth: Culturing methods, including media preparation and sterilization techniques, are fundamental for isolating and growing microorganisms under controlled laboratory conditions.
Molecular Techniques: Advances in molecular biology have revolutionized microbiology, allowing for the detection, quantification, and genetic characterization of microorganisms through methods like PCR (Polymerase Chain Reaction), DNA sequencing, and genomics.
Microbial Analysis in Various Environments: Microbiological methods are applied in diverse settings, including clinical laboratories for diagnosing infections, food industry for quality control, environmental monitoring for assessing microbial diversity, and pharmaceutical industry for developing microbial-based products.

10 days (70 working hours)

Module I

Theory sessions

Practical sessions

Training on different methods of sterilisation & decontamination – 6 hours
Training on preparation of media – 6 hours
Training on plating, spreading and culturing methods – 20 hours
Training on microbial identification methods – 12 hours
Training on antimicrobial activity assays – 8 hours
Training on any microbial enzyme activity screening method – 8 hours

One month (144-150 working hours)

Module II

Theory sessions 18h

Introduction to Good Laboratory Practice (GLP) –4 hours
Introduction to essential laboratory equipments -basic and uses –6 hours
Introduction to Microbial Culture methods – 6 hours
Introduction to basics of molecular screening methods – 2 hours

Practical sessions – 130h

Training on use of laboratory equipments - 6 hours
Training on different methods of sterilisation & decontamination – 6 hours
Training on preparation of media (Steam & vacuum sterilization methods) – 6 hours
Training on plating, spreading and culturing methods – 20 hours
Training on isolation of pure cultures from any environmental source – 12 hours
Training on microbial identification methods (staining and biochemical) – 32 hours
Training on molecular methods of microbial identification – 12 hrs
Training on antimicrobial activity assays – 18 hours
Training on microbial enzyme activity screening method – 18 hours

Advantages of attending present training modules

Training in microbiological methods offers several advantages:

Critical Thinking and Problem-Solving: Participants will develop critical thinking skills through hands-on experience in designing experiments, troubleshooting techniques, and interpreting results.
Career Opportunities: Proficiency in microbiological methods enhances career prospects in research institutions, biotechnology companies, pharmaceutical firms, clinical laboratories, and public health agencies.
Contribution to Scientific Advancements: Understanding microbiological methods contribute to advancements in medicine, agriculture, and environmental sustainability by addressing challenges such as antibiotic resistance, food safety, and microbial biotechnology.

This training course is structured to provide a blend of theoretical knowledge and practical skills necessary to conduct microbiological research effectively. Participants will engage in laboratory exercises, demonstrations, and discussions aimed at building a strong foundation in microbiological methods.